Morpho-functional response of Nile tilapia (Oreochromis niloticus) to a homeopathic complex.
IACH - Prof. G. Vithoulkas
Morpho-functional response of Nile tilapia (Oreochromis niloticus) to a homeopathic complex.
  • Author(s)

    Braccini GL, et al.

    Journal

    Homeopathy, 2013, 102, 4, 233-41.

    Notes

    This work looked at the effects of different concentrations of a homeopathic complex (Homeopatila 100) on the performance and prevalence of ectoparasites and the morpho-functional response of the liver and the branchiae of the fish, Nile tilapia (Oreochromis niloticus). This trial used farmed juvenile Nile tilapia that were randomly assigned to receive one of four treatments: negative control or 20 mL, 40 mL or 60 mL of the homeopathic combination per kg of fish meal food. There were four replications per treatment type over a period of 57 days. Assessment was via the number of hepatocytes/mm, the intercellular glycogenic behaviour, the rates of histological change (hyperplasia, lamella fusion and telangiectasia) and the percentage of neutral and acidic mucin-producing cells. Significant effect differences were found between the different homeopathic combination treatments and the hepatosomatic ratio of fish treated with it was significantly lower than that of fish from the control group. The best results in the liver and branchiae were achieved with a concentration or 40mL per kg of fish meal.

    Abstract

    BACKGROUND:

    This study evaluated the performance, prevalence of ectoparasites and morpho-functional response of the liver and the branchiae of Nile tilapia (Oreochromis niloticus) raised on fish meal with added of the homeopathic complex Homeopatila 100(®) at different concentrations.

    METHODS:

    Post-reversed juvenile Nile tilapia (O. niloticus) of the GIFT (Genetic Improvement of Farmed Tilapia) strain were used in this study. The performance, ectoparasite prevalence and parasite load in the branchiae and skin as well as the liver and branchial histology. Fish were randomly assigned to receive one of four treatments: control, 20 mL hydroalcoholic solution (alcohol 30° GL); 20 mL Homeopatila 100(®) per kg of meal; 40 mL Homeopatila 100(®) per kg of meal; or 60 mL of Homeopatila 100(®) per kg of meal, compared to control with out the addition of the complex. There were four replications per treatment type (16 experimental units total) at a density of 40 fish per m(3) over a period of 57 days. The Kruskal-Wallis H test (p < 0.05) was employed to analyse the physical and chemical parameters of water as well as for parasite prevalence; whereas analysis of variance was used for liver performance. If the values were significant (p < 0.05), they were compared by Tukey's test. Multiple comparisons of averages were performed using Student's t test (p < 0.05).

    RESULTS:

    There were no significant between the physical and chemical parameters of the water between the different groups at the end of the experiment. Significant differences (p < 0.05) in the mixed parasite conditions were found within the different Homeopatila 100(®) treatments. The hepatosomatic ratio of fish treated with Homeopatila 100(®) was significantly lower than that of fish from the control group. The best results in the liver and branchiae occurred in fish receiving Homeopatila 100(®) at 40 mL/kg in terms of the number of hepatocytes/mm(2), the intercellular glycogenic behaviour, the rates of histological changes (hyperplasia, lamella fusion and telangiectasia) and the percentage of neutral and acidic mucin-producing cells.

    CONCLUSION:

    The addition of Homeopatila 100(®) at a concentration 40 mL per kg/meal to the diet of juvenile Nile tilapias resulted in improved hepatocytes and intracellular glycogen levels as well as the lowest mean rate of branchial histological changes with an increase in acidic mucin-producing cells compared to neutral mucin-producing cells, compared to control.

    Category: Animal Studies

    External url

    https://www.ncbi.nlm.nih.gov/pubmed/24050768

input:focus, select:focus, textarea:focus { border-color: #bd2626; } a { color: #bd2626; } .radio i, .checkbox i { color: #bd2626; } .radio i:before { background-color: #bd2626; } .button, .btn-default { border-color: #bd2626; background-color: #bd2626; } .button:hover { color: #bd2626; } .page-header-top { background-color: #bd2626; } .page-header-bottom-alt { background-color: #bd2626; } .page-intro:after { background-color: #bd2626; } .page-intro:before { background-color: #bd2626; } .page-content-section-bg { background-color: #bd2626; } .page-content-section-bg:before { background-color: #bd2626; } .page-content-section-border { border-top-color: #bd2626; } .page-footer { background-color: #bd2626; } .block-head-1 { border-bottom-color: #bd2626; } .block-head-1 i { background-color: #bd2626; } .block-head-2:after { background-color: #bd2626; } .block-head-2 i:after, .block-head-2 i:before { background-color: #bd2626; } .block-head-3 { border-bottom-color: #bd2626; } .block-head-3 i:after { background-color: #bd2626; } .block-head-4:after { background-color: #bd2626; } .block-head-7 { border-bottom-color: #bd2626; } .block-head-7:after { background-color: #bd2626; } .page-content-section-bg .block-head-3 i { color: #bd2626; } .main-search button { color: #bd2626; } .main-search button:after { background: #bd2626; } .main-nav li a { color: #bd2626; } .main-nav li a:before { background-color: #bd2626; } .main-nav li ul { background-color: #bd2626; } .main-nav li li:hover > a, .main-nav li li.active > a, #block-newsletter-newsletter-subscribe .form-submit:hover, .block-product-details .add-wishlist .button-alt:hover, .block-product-details-2 .add-wishlist .button-alt:hover { color: #bd2626; } .main-nav .switcher { border-color: #bd2626; background-color: #bd2626; } .main-nav .mega > div { background-color: #bd2626; } #block-newsletter-newsletter-subscribe .form-submit, .btn-primary:hover, .btn-primary:focus, .btn-primary:active { border-color: #bd2626; background-color: #bd2626; } .art-portfolio ol li.active, .ui-slider-horizontal .ui-slider-handle, .ui-slider-horizontal .ui-slider-range, .btn-primary.link { background-color: #bd2626; } .btn-primary, .button:hover, .btn-default:hover, .btn-default:focus, .btn-default:active { border-color: #bd2626; color: #bd2626; } .btn-primary.link:hover, .btn-primary.link:focus, .btn-primary.link:active { color: #bd2626; } } @media screen and (max-width: 767px) { /* phone */ .main-nav ul { background-color: #bd2626; } .main-nav li a { color: #ecf0f1; } .main-nav li ul { background: #e9e9e9; } .main-nav li li { border-top-color: #bd2626; } .main-nav li li a { color: #bd2626; } .main-nav li li a:hover { background-color: #bd2626; color: #fff; } .main-nav li li ul { border-top-color: #bd2626; } .main-nav .mega ul li { border-top-color: #bd2626; } } .slider-revolution .fa:after { background-color: #bd2626; } .slider-revolution .title { border-top-color: 1px solid #bd2626; border-bottom-color: 1px solid #bd2626; } .slider-revolution .button-my { border-color: #bd2626; background-color: #bd2626; } .slider-revolution .button-my:hover { color: #bd2626; } .pagination-1 a:hover { border-color: #bd2626; color: #bd2626; } .pagination-1 .active, .pagination-1 .active:hover { border-color: #bd2626; background: #bd2626; } .pagination-2 a:hover { color: #bd2626; } .pagination-2 .active, .pagination-2 .active:hover { background: #bd2626; } .pagination-3 a:before { box-shadow: inset 0 0 0 1px #bd2626; } .pagination-3 .active:before, .pagination-3 .active:hover:before { background: #bd2626; } .pagination-4 a:hover { border-color: #bd2626; } .pagination-4 .active { border-color: #bd2626; background: #bd2626; } .pagination-5 a { border-color: #bd2626; background-color: #bd2626; color: #bd2626; } .block-benefits div:hover { border-color: #bd2626; background-color: #bd2626; } .block-benefits i { color: #bd2626; } .block-benefits-2 li i { color: #bd2626; } .block-benefits-2 .tabs a:hover { border-color: #bd2626; background-color: #bd2626; } .block-benefits-2 .tabs .active a, .block-benefits-2 .tabs .active a:hover { color: #bd2626; } .block-benefits-3 i { color: #bd2626; } .block-about .button { border-color: #bd2626; color: #bd2626; } .block-about .button:hover { background: #bd2626; color: #ecf0f1; } .block-about .owl-prev:hover, .block-about .owl-next:hover { color: #bd2626; } .block-about-2 .bar { background: #bd2626; } .block-about-2 .value { color: #bd2626; } .block-about-4 .owl-pagination div { border-color: #bd2626; background: #bd2626; } .block-about-5 .owl-prev, .block-about-5 .owl-next { display: inline-block; border-color: #bd2626; background-color: #bd2626; } .block-about-5 .owl-prev:hover, .block-about-5 .owl-next:hover { color: #bd2626; } .block-about-6 .icons a:hover { color: #bd2626; } .block-mission .year { background: #bd2626; } .block-mission .years a:after { border-bottom-color: #bd2626; } .block-recent-works ol li:hover { color: #bd2626; } .block-recent-works ol .active { border-color: #bd2626; background: #bd2626; } .block-recent-works .description { background: #bd2626; } .block-recent-works .description:before { border-right-color: #bd2626; } .block-recent-works-3 h3 { border-top-color: #bd2626; } .block-recent-works-3 .link { border-color: #bd2626; } .block-recent-works-3 .fancybox { background-color: #bd2626; } .block-recent-works-3 .owl-pagination div { border-color: #bd2626; background: #bd2626; } .block-recent-works-4:before { background: #bd2626; } .block-recent-works-4 .fancybox { background: #bd2626; } .block-recent-works-4 .owl-prev:hover, .block-recent-works-4 .owl-next:hover { color: #bd2626; } .block-recent-works-6 a:before { background: #bd2626; } .block-recent-works-6 .owl-prev, .block-recent-works-6 .owl-next { border-color: #bd2626; background: #bd2626; } .block-recent-works-6 .owl-prev:hover, .block-recent-works-6 .owl-next:hover { color: #bd2626; } .block-portfolio ol li:hover { color: #bd2626; } .block-portfolio ol li.active { border-bottom-color: #bd2626; } .block-portfolio ul .pic span:after, .block-portfolio ul .pic span:before { background: #bd2626; } .block-portfolio ul .pic span i { color: #bd2626; } .block-portfolio-2 ol li:hover { color: #bd2626; } .block-portfolio-2 ol li.active { background: #bd2626; } .block-portfolio-2 ul .pic:before { background: #bd2626; } .block-portfolio-2 ul .pic a { background: #bd2626; } .block-portfolio-2 ul .pic a:hover { color: #bd2626; } .block-portfolio-3 ol li:hover { color: #bd2626; } .block-portfolio-3 ol li.active { background: #bd2626; } .block-portfolio-4 h3 { border-top-color: #bd2626; } .block-portfolio-4 .link { border-color: #bd2626; } .block-portfolio-4 .fancybox { background: #bd2626; } .block-portfolio-5 ol li:hover { color: #bd2626; } .block-portfolio-5 ol li.active { background: #bd2626; } .block-portfolio-5 .info:before { background: #bd2626; } .block-portfolio-6 ol li:hover { color: #bd2626; } .block-portfolio-6 ol li.active { background: #bd2626; } .block-portfolio-6 ul a:before { background: #bd2626; } .block-portfolio-7 ol li:hover { color: #bd2626; } .block-portfolio-7 ol li.active { background: #bd2626; } .block-portfolio-7 ul li:before { background: #bd2626; } .block-portfolio-7 ul a:hover { color: #bd2626; } .block-portfolio-8 li div:before { background: #bd2626; } .block-portfolio-8 li a { background: #bd2626; } .block-clients .owl-prev, .block-clients .owl-next { color: #bd2626; } .block-testimonials-3 .carousel h3 { border-bottom-color: #bd2626; } .block-testimonials-3 .owl-pagination div { border-color: #bd2626; } .block-testimonials-3 .owl-pagination .active { background: #bd2626; } .block-testimonials-4 .owl-prev, .block-testimonials-4 .owl-next { border-color: #bd2626; background: #bd2626; } .block-testimonials-4 .owl-prev:hover, .block-testimonials-4 .owl-next:hover { color: #bd2626; } .block-services li a { border-color: #bd2626; } .block-services li a:hover { background-color: #bd2626; } .block-services-2 li a:hover { background: none; } .block-services-2 li a:after { box-shadow: inset 0 0 0 2px #bd2626; } .block-services-2 li a:hover:after { background-color: #bd2626; } .block-services-3 li a:hover { background: none; } .block-services-3 li a:after { box-shadow: inset 0 0 0 2px #bd2626; } .block-services-3 li a:before { background: #bd2626; box-shadow: inset 0 0 0 2px #bd2626; } .block-services-3 li a:hover:after { background-color: #bd2626; } .block-services-4 li a { border-color: #bd2626; } .block-services-4 li a:hover { background-color: #bd2626; } .block-services-5 li i { color: #bd2626; } .block-services-5 li i:hover { background: #bd2626; } .block-services-6 h3 span { color: #bd2626; } .block-services-6 li a { border-color: #bd2626; background: #bd2626; } .block-services-6 li a:hover { color: #bd2626; } .block-services-7 .info i { background: #bd2626; } .block-services-7 .carousel .active { background: #bd2626; } .block-services-7 .carousel .owl-prev, .block-services-7 .carousel .owl-next { border-color: #bd2626; background: #bd2626; } .block-services-7 .carousel .owl-prev:hover, .block-services-7 .carousel .owl-next:hover { color: #bd2626; } .block-pricing dt { color: #bd2626; } .block-pricing .price:before { background: #bd2626; } .block-pricing li:hover .inner { background: #bd2626; } .block-pricing li:hover .button { color: #bd2626; } .block-pricing li:hover .button:hover { background: #bd2626; } .page-content-section .block-pricing .inner { box-shadow: 0 0 0 1px #bd2626; } .block-pricing-2 dt { color: #bd2626; } .block-pricing-2 .price { background: #bd2626; } .block-pricing-2 .button { color: #bd2626; } .block-pricing-2 li:hover .inner { background: #bd2626; } .block-pricing-2 li:hover .button:hover { background: #bd2626; } .block-progress li i { border-color: #bd2626; color: #bd2626; } .block-team-2 .pic:before { background-color: #bd2626; } .block-team-2 .icons a { color: #bd2626; } .block-team-3 .pic:before { background-color: #bd2626; } .block-team-3 .icons a { color: #bd2626; } .block-team-3 .info h3 { color: #bd2626; } .block-team-list .pic span:after { background-color: #bd2626; } .block-team-list .icons a:hover { color: #bd2626; } .block-team-list .icons a:before { background: #bd2626; } .block-team-list .skills { border-top-color: #bd2626; border-bottom-color: #bd2626; } .block-team-grid .pic:before { background-color: #bd2626; } .block-skills i:before { background-color: #bd2626; } .block-skills .bar { border-color: #bd2626; background-color: #bd2626; } .block-skills-2 i:before { background-color: #bd2626; } .block-skills-2 .bar { border-color: #bd2626; background-color: #bd2626; } .block-capabilities h3 { color: #bd2626; } .block-capabilities-2 h3 { color: #bd2626; } .block-recent-posts .info { background-color: #bd2626; } .block-recent-posts-2:before { background-color: #bd2626; } .block-recent-posts-2 li .button { color: #bd2626; } .block-recent-posts-2 li .button:hover { background-color: #bd2626; } .block-recent-posts-2 .button-more { color: #bd2626; } .block-recent-posts-2 .button-more:hover { background-color: #bd2626; } .block-featured-posts .pic:before { background-color: #bd2626; } .block-blog-list .link { background: #bd2626; } .block-blog-list .date:before { background-color: #bd2626; } .block-blog-list .date-alt { background-color: #bd2626; } .block-blog-list .zoom { background-color: #bd2626; } .block-blog-grid .link { background: #bd2626; } .block-blog-grid .date:before { background-color: #bd2626; } .block-blog-grid .pic:hover .date { color: #bd2626; } .block-blog-grid .zoom { color: #bd2626; } .block-blog-details .date:before, .block-blog-details .reply:before, .block-blog-details .type:before { background-color: #bd2626; } .block-blog-details .date-alt { background-color: #bd2626; } .block-blog-details .share a:before { border-color: #bd2626; background: #bd2626; } .block-blog-details .share a:hover { color: #bd2626; } .block-blog-details .about:before { background: #bd2626; } .block-catalog-toolbar .view a:hover { border-color: #bd2626; color: #bd2626; } .block-catalog-toolbar .view .active, .block-catalog-toolbar .view .active:hover { border-color: #bd2626; background: #bd2626; } .block-catalog-toolbar .direction { border-color: #bd2626; background: #bd2626; } .block-catalog-toolbar .direction:hover { color: #bd2626; } .block-catalog-grid .pic:before { background: #bd2626; } .block-catalog-grid .badge { background: #bd2626; } .block-catalog-grid .price { color: #bd2626; } .block-catalog-grid .owl-prev, .block-catalog-grid .owl-next { border-color: #bd2626; background: #bd2626; } .block-catalog-grid .owl-prev:hover, .block-catalog-grid .owl-next:hover { color: #bd2626;; } .block-catalog-list .pic:after { background-color: #bd2626; } .block-catalog-list .badge:before { background-color: #bd2626; } .block-catalog-list .price { color: #bd2626; } .block-catalog-list .owl-prev, .block-catalog-list .owl-next { border-color: #bd2626; background-color: #bd2626; } .block-catalog-list .owl-prev:hover, .block-catalog-list .owl-next:hover { color: #bd2626;; } .block-product-details .price { color: #bd2626; } .block-product-details .button-alt { color: #bd2626; } .block-product-details-2 .button-alt { color: #bd2626; } .block-product-details-2 .share a:before { background-color: #bd2626; box-shadow: 0 0 0 2px #bd2626; } .block-product-details-2 .share a:hover { color: #bd2626; } .block-product-info .owl-prev, .block-product-info .owl-next { border-color: #bd2626; background-color: #bd2626; } .block-product-info .owl-prev:hover, .block-product-info .owl-next:hover { color: #bd2626; } .block-product-tabs .head .active { color: #bd2626; } .block-product-tabs .head .active:before { border-top-color: #bd2626; } .block-shopping-cart th { border-top-color: #bd2626; color: #bd2626; } .block-shopping-cart .price { color: #bd2626; } .block-shopping-cart .remove { border-color: #bd2626; background-color: #bd2626; } .block-shopping-cart .remove:hover { color: #bd2626; } .block-shopping-cart .quantity a { color: #bd2626; } .block-shopping-cart .empty td { border-bottom-color: #bd2626; } .block-shopping-cart-totals dd { color: #bd2626; } .block-shopping-cart-totals .button-alt { color: #bd2626; } .block-checkout-order td:last-child { color: #bd2626; } .block-checkout-payment label { color: #bd2626; } .block-welcome .icons i:after { border-color: #bd2626; background: #bd2626; } .block-welcome .icons a:hover i { color: #bd2626; } .block-pasteboard:before { background: #bd2626; } .block-pasteboard .icons a:hover { color: #bd2626; } .block-map-3 .block-head { color: #bd2626; } .block-contacts ul li:after, .block-contacts ul li:before { border-color: #bd2626; background-color: #bd2626; } .block-contacts ul li:before { background-color: #fff; } .block-contacts ul li span { border-bottom-color: #bd2626; } .block-contacts ul .active { color: #bd2626; } .block-contacts ul .active:before { background-color: #bd2626; } .block-contacts ol { border-top-color: #bd2626; } .block-contacts ol li { color: #bd2626; } .block-contacts ol li i:after { background-color: #bd2626; } .block-feedback .message i, .block-feedback-2 .message i { color: #bd2626; } .block-not-found h3 { color: #bd2626; } .block-not-found .pic:before { background-color: #bd2626; } .block-not-found .pic div:before { background-color: #bd2626; } .block-not-found-2 .button { color: #bd2626; } .block-not-found-2 .button:hover { background: #bd2626; } .block-coming-soon li:before { background-color: #bd2626; } .block-coming-soon-2 li:after { background-color: #bd2626; } .widget-categories a:hover, .widget-categories a.active { color: #bd2626; } .widget-top-posts a:hover { color: #bd2626; } .widget-text hr { border-top-color: #bd2626; } .widget-text em { color: #bd2626; } .widget-archive a:hover, .widget-archive a.active { color: #bd2626; } .widget-tags a:hover { color: #bd2626; } .widget-categories-filter a:hover { color: #bd2626; } .widget-categories-filter .active { color: #bd2626; } .widget-categories-filter .active span { background-color: #bd2626; } .widget-price-filter .ui-slider-range { background-color: #bd2626; } .widget-price-filter .ui-slider-handle { background-color: #bd2626; } .widget-color-filter .active:before { border-color: #bd2626; } .widget-top-products .pic i { background-color: #bd2626; } .main-nav ul { background-color: #bd2626; }