Influence of very low doses of mediators on fungal laccase activity – nonlinearity beyond imagination.
IACH - Prof. G. Vithoulkas
Influence of very low doses of mediators on fungal laccase activity – nonlinearity beyond imagination.
  • Author(s)

    Malarczyk E, J , Kochmanska-Rdest , Jarosz-Wilkolazka A.

    Journal

    Nonlinear Biomedical Physics 2009, 3, 10.

    Notes

    Carried out at the Biochemistry Department, Maria Curie-Skłodowska University, Lublin, Poland, this trial was conducted by Elzbieta Malarczyk and her colleagues to determine the effect of various homeopathically potentised substances (1C to 10C) hydroxybenzotriazole (HBT) and 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) ), on the activation of extracellular Laccase, an enzyme with industrial application that’s responsible for the aerobic transformations of some natural phenolics. HBT and ABTS at material dose are known laccase activators. The experiment was done by exposing 2 high laccase fungal strains to 1C to 10C potencies of HBT and ABTS over 14 days, the laccase produced assessed for activity via PAGE and colorimetric tests and the level of demethylation activity assessed after incubation with veratric acid. On all assessments the potentised versions of HBT and ABTS demonstrated a statistically significant effect.

    Abstract

    Laccase, an enzyme responsible for aerobic transformations of natural phenolics, in industrial applications requires the presence of low-molecular substances known as mediators, which accelerate oxidation processes. However, the use of mediators is limited by their toxicity and the high costs of exploitation. The activation of extracellular laccase in growing fungal culture with highly diluted mediators, ABTS and HBT is described. Two high laccase-producing fungal strains, Trametes versicolor and Cerrena unicolor, were used in this study as a source of enzyme. Selected dilutions of the mediators significantly increased the activity of extracellular laccase during 14 days of cultivation what was distinctly visible in PAGE technique and in colorimetric tests. The same mediator dilutions increased demethylation properties of laccase, which was demonstrated during incubation of enzyme with veratric acid. It was established that the activation effect was assigned to specific dilutions of mediators. Our dose-response dilution process smoothly passes into the range of action of homeopathic dilutions and is of interest for homeopaths.

    Category: In Vitro Studies

    External url

    https://www.ncbi.nlm.nih.gov/pubmed/19732425

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