Therapeutic Effect of Arsenicum album on Leukocytes.
IACH - Prof. G. Vithoulkas
Therapeutic Effect of Arsenicum album on Leukocytes.
  • Author(s)

    Ive EC, Couchman IM, Reddy L.

    Journal

    Int J Mol Sci, 2012, 13, 3, 3979-87

    Notes

    Scientists from South Africa’s Durban University of Technology performed this in vitro study to determine the effects of various succussed and unsuccussed homeopathically prepared Arsenic trioxide (Arsenicum album) on human T lymphocytes grown in tissue culture. Cells were intoxicated with arsenic trioxide and then exposed to succussed and unsuccussed Arsenicum 6C, succussed and unsuccussed Arsenicum 30C, succussed and unsuccussed Arsenicum 200C or a control substance for 24, 48 or 72 hours. Cells were assessed for viability at baseline and at these three data points. After the counts from these data points were assessed it was shown that when compared to controls, all of the homeopathically prepared Arsenicum potencies provided a statistically significant increase in cell viability. These results were unaffected by whether the material was succussed or unsuccussed, but were affected by the length of exposure time to the remedy.

    Abstract

    The therapeutic effects of homoeopathic Arsenicum album potencies were investigated in-vitro, using a continuous cell line (MT4), pre-intoxicated with arsenic trioxide (As2O3), and then treated with succussed and unsuccussed homoeopathic potencies, 6CH, 30CH and 200CH. This study aimed to verify the homoeopathic law of similars and to determine whether potencies diluted beyond Avogadro’s constant had physiological effects on cells; whether various potencies would cause different effects as suggested by the concept of hormesis; whether succussed and unsuccussed homoeopathic potencies had different effects on the cells; and to establish whether a biotechnological method could be used to evaluate the above. As2O3 was used to pre-intoxicate and the MTT assay was used to measure the percentage cytotoxicity and half maximal inhibitory concentration (IC50) of the cells. The homoeopathic potencies of Arsenicum album (6CH, 30CH and 200CH) were prepared by either succussing or allowing to diffuse for 30 s. After pre-intoxication of the MT4 cells with the IC50 As2O3 and treatment with succussed and unsuccussed Arsenicum album (6CH-200CH), the cell viability increased with increasing potency from 81% to 194% (over 72 h). The treatments and the times of exposure were found to be statistically significant determinants of cell viability, whereas succussion did not cause any significant variation in the results. The study provided evidence that a biotechnological method (namely cell viability) may be used to scientifically evaluate the physiological effects of homoeopathic potencies on human cells; it confirmed that the homoeopathic potencies did have therapeutic effects; and that succussion was not required in the potentization method in order to produce a curative remedy.

    Category: In Vitro Studies

    External url

    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3317753/

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